2015 - present - "The role of Na+/Ca2+ exchange in reverse mode in regulation of cell hydration"
Conclusion: The dysfunction of cAMP-dependent Na+/Ca2+ exchange in reverse mode is a primary mechanism for age-dependent cardio-muscle failure and memory impairment.
2014 - present - "The role of age-dependent neuronal dehydartion in memory impairment"
Conclusion: The dysfunction of intracellular signaling system controlling cell hydration is responsible for age-dependent depression of neuronal function.
2013 - present - “Bioremediation as a modern and efficient method for water pollution management”
Conclusion: H2O2-induced bactericide properties are elevated by non-critical concentration of CO2.
2013 - present – “Agrobiotechnology and food safety”
Conclusion 1: Extremely low frequency of electromagnetic field (ELF EMF)-treated water as a tool for the increase of growth and development of microbes and plants.
Conclusion 2: ELF EMF treatment as a novel method for the activation of bull sperm motility.
Conclusion 3: Double frequency of impedancemetric characteristics of meat is a novel method for determining its quality.
2010 - present –“The role of dysfunction of α3 isoform-dependent signaling system controlling cell hydration in cancer generation”
Conclusion: The dysfunction of α3 isoform of Na+/K+ pump is a primary mechanism for carcinogenesis.
2010 – present - “The study of the mechanism of age-dependent magneto- and microwave- sensitivity of rat brain and heart muscles in norm and pathology”
Conclusion: Age-dependent dysfunction of Na+/K+ pump α3 isoform-dependent signaling system controlling cell hydration for the decrease of magnetosensitivity of heart muscle and brain tissue hydration.
2009 - present - “The study of functional role of different Na+/K+ pump isoforms in regulation of cell hydration in norm and pathology”
Conclusion: α3 isoform-dependent signaling system serves as a universal sensor through which the biological effect of weak environmental signals on cells and organisms are realized.
2008 - present – “Cellular and Molecular Mechanism of Biological Effect of Background Ionizing Radiation, Electro Magnetic Fields and Infrasound Frequency of Mechanical Vibration on Plants, Microbes, Invertebrate and Vertebrate Organisms”
Conclusion: Cell bathing aqua medium serves as one of the essential primary targets for biological effects of ionizing and non-ionizing radiation.
2007-present – “The role of the dysfunction of Na+/K+ Pump in age-dependent medical disorders”
Conclusion: Age-dependent dysfunction of α3 isoform-dependent signaling system controlling cell hydration serves as a primary mechanism for generation of age-related medical disorders.
2007-present - “The Correlation Between Brain Cell Hydration and Pain Sensation in mammals”
Conclusion: Cell over-hydration promotes the nociceptive signals generation while dehydration has pain relief and anesthetic effects on mammals.
2007-present - “The Study of Cell Hydration as a Marker for Biological Effect of Environmental Factors”
Conclusion: Cell hydration serves as a universal and extrasensitive sensor for environmental factors in plants, microbes and mammals.
2001-present – “The Study of dependency of cell metabolic activity on cell bathing aqua structure”
Conclusion: - The environmental factor-induced changes in both water molecules disassociation and peroxide formation in cell bathing medium serve as messengers through which cell bathing medium could modulate its metabolic activity.
1998-2009 – “The study of low frequency of EMF and infrasound (IS) on physicochemical properties of water and peroxide formation”.
Conclusion: EMF and IS have 4 and 8Hz frequency “windows” at which they have more pronounced effects on water molecules dissociation, heat fusion, electrical conductivity, gases solubility and hydrogen peroxide formation in water.
1997-2005 – “The nature of metabolic mechanism through which extremely weak chemical and physical factors can modulate conductive function (excitability and chemo sensitivity) of membrane"
Conclusion: cGMP/cAMP dependent-Na:Ca exchanger serves as a universal and extrasensitive membrane sensor for extremely low concentrations of biologically active substances and weak physical factors.
1994- 2009 – “The role of structural changes of cell bathing aqua medium in realization of biological effects of non-ionizng radiation (electromagnetic fields and infrasound) on cells and organisms”.
Conclusion: EMF-induced changes of physicochemical properties and formation of ROS serve as main mechanisms through which non-thermal biological effects of magnetic and electrical fields on cells and organisms are realized.
1994-1997 - “The metabolic mechanism of the effect of NO on heart muscle contractility” (In collaboration with prof. Robert Walker, UK)
Conclusion: NO-induced heart muscle relaxation is due to the activation of cGMP-dependent Ca2+ efflux and Na+/Ca2+ exchange in forward mode from the cells.
1994-1997 – “The correlation between Na+/Ca2+ exchange and intracellular cAMP/cGMP”
Conclusion: Intracellular cGMP plays a key role in the activation of Ca efflux through Na+/Ca2+exchange in forward mode and Ca pump mechanisms, while intracellular cAMP activates Ca influx by
Na+/Ca2+ exchange in reverse mode.
1990-1998 - “Biological effect of extremely low concentrations of transmitters on membrane functional activity”. (In collaboration with David Carpenter, USA, Robert Walker, UK, Peter Usherwood, UK, Yanosh Salanki, Hungary)
Conclusion: Low concentration having no effect on membrane conductive properties regulate the conductive function of membrane through modulatation of intracellular signaling system.
1989-1990 – “Metabolic regulation of N-cholinoreceptor affinity”
Conclusion: Inactivation of Na-pump leads to the increase of number of functionally active chemoreceptors in membrane and the decrease of their affinity to ligands.
1988-1989 – “Correlation between Na+/K+ pump activity and intracellular contents of cAMP”
Conclusion: Pump inactivation leads to the increase of intracellular cAMP contents while its activation has the opposite effect.
1987 – 1991 - “The study of the functional role of Na+/K+ pump in regulation with cell sensitivity to ionizing radiation” (in collaboration with Dvoretsky A.I., Shainskaya A.M., Ukraine)
Conclusion: Ionic radiation-induced Na+/K+ pump inhibition leads to the increase of the number of pump units in the membrane. High affinity ouabain receptors have higher sensitivity to ionizing radiation than low affinity receptors.
1986-1988 – “Correlation between Na+/K+ pump activity and Na+/Ca2+ exchange”
Conclusion: There is a negative correlation between Na+/K+ pump and Na+/Ca2+ exchange activity that is realized by intracellular level of cAMP.
1984-1992 - “The study of the role of lipid composition in regulation of membrane protein function” (In collaboration with Prof. Toshifumi Takenaka, Japan)
Conclusion: Short-chain fatty acids can be effective modulators of both ouabain-sensitive and ouabain-insensitive fractions of Na+ efflux from the cells, membrane excitability and chemosensitivity.
1984-1991 – “The ionic membrane mechanism of ionizing radiation on neuromembrane function”.
Conclusion: Ca2+-dependent activation of Lipase A2 activity is the gate of ionizing radiation of membrane damage (In collaboration with prof. Konstantin Karageuzyan, Armenia)
1980-1987 “The role of membrane lipid surrounding in regulation the functional activity of membrane protein”
Conclusion: The negative correlation between Na+/K+ pump and Na+/Ca2+ exchange depends on membrane lipids’ fluidity. The increase of the latter leads to disappearance of the mentioned correlation. Decanoid acid- induced membrane fluidity leads to the activation of the agonist-induced K+ currents.
1980-1988 - “The effect of water fluxes through the membrane on transmembrane characters” (In collaboration with Prof. Kiozo Koketsu, Japan)
Conclusion: Transmembrane water fluxes having activation effect on membrane current have the same direction and inactivation effect on current having opposite direction.
1977-1984 – “The study of the functional role of electrogenic Na+/K+ pump in regulation of cell volume”
Conclusion: Na+/K+ pump has a cell volume regulation function: its activation leads to the shrinkage while inactivation to cell swelling.
1980-1990 – “The study of functional role of pump-induced cell volume changes in regulation of membrane chemo sensitivity and excitability”
Conclusion: Number of functionally active protein molecules having enzymatic chemoreceptive and ion channel forming properties depend on cell surface: surface increasing leads to elevation of number of functional active proteins while shrinkage has the opposite effect.
1969-1976 – “The mechanism regulating pacemaker activity of Helix neurons” (In collaboration with prof. Howard Wachtell, USA)
Conclusion: The oscillation of electrogenic sodium pump-dependent cell volume is responsible for membrane potential oscillation of pacemaker neurons.
1965-1969 – “The study of the role of cell metabolic processes in generation of membrane potential of snail neuron”. (postgraduate thesis, advisor- Prof. Platon Kostyuk, Ukraine)
Conclusion: The resting potential of snail neurons consists of two components of different nature: the first is purely diffused, and is due to the presence of ionic gradient on the membrane, the second is generated by electrogenic sodium pump.